Description
The principle of the following chemiluminescence immunoassay (LUM) test follows a two-step competitive binding scenario. Competition occurs between an unlabeled antigen (present in calibrators, control and unknowns) and a biotin-labelled antigen for a limited number of anti-estrone antibody binding sites on the microwell plate. After washing the streptavidin-horseradish peroxidase conjugate is incubated and forms a complex with the bound biotinylated estrone. The washing and decanting procedures remove unbound materials, and then luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of estrone in the unknown. A set of calibrators is used to plot a calibration curve from which the amount of estrone in saliva unknowns and controls can be directly read